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beta-galactosidase preparation and assays (18)Beta-glucuronidase (27)
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Lab Book Ref
JRH4712-63 (1)JRH4712-64 (2)
JRH4712-66 (1)
Jrh4712-76 (1)
Jrh4712-77 (1)
Jrh4712-78 (1)
Jrh4712-80 (1)
Jrh4712-81 (1)
Jrh4712-82 (1)
Jrh4712-83 (1)
Jrh4712-84 (1)
Jrh4712-85 (1)
4712-88 (1)
Jrh4712-89 (1)
4712-86 (1)
Jrh4712-87 (1)
4712-90a (1)
4712-91 (1)
4712-94 (1)
Product
Jrh4712-74 (1)Jrh4712-76 (1)
Jrh4712-76a (1)
Sample Parent
Jrh4712-74 (1)Jrh4712-76 (1)
Jrh4712-76a (1)
Jrh4712-77 (1)
Jrh4712-79 (1)
Jrh4712-80 White (1)
Jrh4712-82 (1)
Jrh4712-80 Blue (2)
4712-86 (1)
4712-84 Beta-gal (1)
4712-80 Blue (1)
4712-88 (1)
4712-93 (1)
Sample Parent2
Jrh4712-80 Blue (1)Jrh4712-80 White (2)
4712-84 PBad (1)
4712-80 White (1)
Sample Parent 3
4712-74 (1)Post Type
Enzyme (5)Buffer (1)
Template (1)
Ligation Product (1)
Tools
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Post Type: Ligation_product
10 μL of ligation reaction.
10 μL of ligation reaction.
Post Type: Template
Ligation reactions were set up in 200 μL tubes and incubated as per the given conditions. The products were kept for the next process without purification.
| Reagent | Reagent Metadata | Reaction Amount (μL) |
| Backbone | links to lists of possible digestion products | |
| Insert | links to lists of possible digestion products | |
| Buffer | links to lists of possible buffers | |
| Enzyme | links to lists of possible enzymes | |
| Ligation temperature | ||
| Ligation time | ||
| links to product post |
Ligation reactions were set up in 200 μL tubes and incubated as per the given conditions. The products were kept for the next process without purification.
Post Type: Buffer
This buffer is MgCl2 free and comes with 25 mM MgCl2 separately.
| Property | Data |
| Name | GoTaq Flexi Reaction Buffer |
| Supplier | Promega |
| Batch Number | 20598205 |
| Expiry | None listed |
| pH | Not provided |
| Ingredients | Not provided |
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| 6 |
This buffer is MgCl2 free and comes with 25 mM MgCl2 separately.
Post Type: Enzyme
| Property | Data |
| Name | T4 DNA Ligase |
| Supplier | Promega |
| Batch Number | 19827724 |
| Expiry | September 2009 |
| Cutting Sequence | N/A |
| Storage Buffer | 10 mM Tris-HCl (pH 7.4 @ 25°C) |
| 2 | 50 mM KCl |
| 3 | 1 mM DTT |
| 4 | 0.1 mM EDTA |
| 5 | 50% Glycerol |
| 6 |
Post Type: Enzyme
| Property | Data |
| Name | GoTaq |
| Supplier | Promega |
| Batch Number | 21489801 |
| Expiry | April 2008 |
| Cutting Sequence | N/A |
| Storage Buffer | Ingredients list not provided |
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| 6 |