Login| Reagent | Reagent Metadata | Reaction Amount (μL) |
| Backbone | links to lists of possible digestion products | |
| Insert | links to lists of possible digestion products | |
| Buffer | links to lists of possible buffers | |
| Enzyme | links to lists of possible enzymes | |
| Ligation temperature | ||
| Ligation time | ||
| links to product post |
| Property | Data |
| Name | GoTaq Flexi Reaction Buffer |
| Supplier | Promega |
| Batch Number | 20598205 |
| Expiry | None listed |
| pH | Not provided |
| Ingredients | Not provided |
| 2 | |
| 3 | |
| 4 | |
| 5 | |
| 6 |
| Property | Data |
| Name | T4 DNA Ligase |
| Supplier | Promega |
| Batch Number | 19827724 |
| Expiry | September 2009 |
| Cutting Sequence | N/A |
| Storage Buffer | 10 mM Tris-HCl (pH 7.4 @ 25°C) |
| 2 | 50 mM KCl |
| 3 | 1 mM DTT |
| 4 | 0.1 mM EDTA |
| 5 | 50% Glycerol |
| 6 |
| Property | Data |
| Name | GoTaq |
| Supplier | Promega |
| Batch Number | 21489801 |
| Expiry | April 2008 |
| Cutting Sequence | N/A |
| Storage Buffer | Ingredients list not provided |
| 2 | |
| 3 | |
| 4 | |
| 5 | |
| 6 |