Beta-Glu

The gel worked really well and as the reaction was very clean, I combined the two reactions and used the direct PCR purification method.
I measured that concentration (the post-PCR mix) on the nano-drop and I'll measure it again now it's been purified. The pre-purification concentration looked healthy so if purification has been successful, it should be a healthy concentration to take into the digestion step.

Unfortunately the purification appears not to have gone well. Though I also can't get any consistency from the figures given by the nano-drop. These are the results I got:

-	reading 1	reading 2	reading 3	reading 4	reading 5	reading 6	average
PCR product before*	282.3 ng/μL	283.4 ng/μL	281.1 ng/μL	N/A	N/A	N/A	282.3 ng/μL
PCR product after*	7.8 ng/μL	12.9 ng/μL	17.6 ng/μL	85.4 ng/μL	22.4 ng/μL	12.8 ng/μL	?

*Both reactions combined together after PCR

I'm going to do another PCR again. That step is working really well. I'm just not sure what to do about purifying it. The only other thing I can try is eluting in TE buffer rather than water (which it says you can also elute into)
In this purification I used preheated water and followed the instructions closely. Perhaps the DNA will elute into TE more effectively.

I would definitely compare these on a gel so as to see whether it is just the nanodrop that is the problem. It might help also if you are explicit about how much solution you are trying to purifiy and what the final volume is.