Investigations into neutral drift

I had been re-writing a section of my thesis today and on going to do an examiner's correction, found that the correct version of several files had not been added to the blog.

However on going to the relevant post and trying to upload data, I was confronted with a problem - the upload data pop-up window is not displaying correctly so I am unable to upload data.

So hopefully someone will see this and correct the problem...

In the meantime the file I was trying to upload can be found here on dropbox:

https://dl.dropbox.com/u/61883354//jrh5025_56%20beta-galactosidase%20activity.xlsx

The file is the kinetic assay for the beta-galactosidase activity of my mutant jrh5025/46.b1. The updated file now contains the updated, corrected graphs that feature in the thesis. They are found under the tab labelled 'Graphs thesis'. The 'reorganised' tab has now been updated to include meaningful data series headings.

A numerical error in the Beer-Lambert calculation used to convert the raw absorbances into concentrations has led to a lot of the data in assay 4712/2 being wrong. I have now corrected the data and updated the graphs. This is now the corrected data file.

It seems I can't update posts and add further data files to the post so another new post. This is the final files 4712/28 and 4712/28a, the final assays.

I think I am up to date now with all the assays. There are 14 in total across 16 files which should all now be up on the blog:

jrh4594/70

jrh4594/72

jrh4594/75

jrh4594/76

jrh4594/78

jrh4594/80

jrh4594/81 (failed)

jrh4594/100

jrh4712/2

jrh4712/7

jrh4712/9

jrh4712/13

jrh4712/24 and jrh4712/24a

jrh4712/28 and jrh4712/28a

I was correlating old computer file names with my lab book and found a couple more unanalysed files. I've now sorted these out and they are attached. That gives a total of 14 assays which generated results files. A further three assays were carried out but these do not have an associated results file remaining. In some cases it has been noted in the paper lab book that the assay had failed.

Neither of the examiners were happy with the way in which I had written my methods section for the beta-glu work. I have now re-written these to try to reflect the style of a paper. However, I have kept them as a numbered list as I have included methods that would not normally be included in a paper as they would be assumed knowledge but probably need spelling out in a thesis.

Any comments on if these have been improved (compared to the version in the original thesis) would be appreciated. Main changes are taking out the table style structure with exact amounts of solutions used, instead changing to molarities of solutions, units of enzymes etc

I think that may have been a by-product of thinking in tables for so long for the blog!