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Post Type: Cell_strain
18 aliquots of ~120 μL of BW25141 in glycerol
The change from lacIq to lacI+ is from openwetware.
18 aliquots of ~120 μL of BW25141 in glycerol
| Property | Data |
| Name | BW25141 electrocompetent cells |
| Number | ESOT028 |
| Organism | E. coli |
| Antibiotic resistance | None |
| Genotype | lacI+, rrnBT14, ΔlacZWJ16, ΔphoBR580, hsdR514, ΔaraBADAH33, ΔrhaBADLD78, galU95, endABT333, uidA(ΔMluI)::pir+, recA1 |
| Plasmid | None |
The change from lacIq to lacI+ is from openwetware.
Linked Posts
This post is linked by:
- Production of BW25141 competent cells (batch 02/08)
- Electroporation and screen of native β-glucuronidase against alternative substrates to compare with 5025/66
- Electroporation and X-gal screen of Wendy's old experiments
- Electroporation and re-screening of Wendy's 1st and 2nd experiment with X-gal
- Electroporation 5025/69 and alternative substrate screening of Wendy's round 4 and round 5 expts and my round 1
- Electroporation 26.05.09 checking whether Wendy's plasmids were selected/unselected libraries
There is currently a corruption of the html coding for the genotype occurring. I will have to go back to the E.coli centre listings to get what the genotype is and correct it.
Anything that is an I with ^ on top followed by " is wrong. I think it should be a delta but will have to check.
;Anything that is an I with ^ on top followed by " is wrong. I think it should be a delta but will have to check.